| Autor: |
Woo, Patrick C.Y., Chong, Ken T.K., Lau, Candy C.Y., Wong, Samson S.Y., Lau, Susanna K.P., Kwok-yung Yuen |
| Předmět: |
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| Zdroj: |
FEMS Microbiology Letters; Sep2006, Vol. 262 Issue 2, p138-147, 10p, 4 Diagrams, 1 Chart, 3 Graphs |
| Abstrakt: |
Using serum from guinea-pigs immunized with a Δ AFMP1Δ AFMP2 deletion mutant of Aspergillus fumigatus to screen a cDNA library of A. fumigatus, we cloned a novel immunogenic 57-kDa protein in A. fumigatus. We also cloned its 55-kDa homologue in Penicillium marneffei, which was possibly related to amino acid biosynthesis and metabolism, with homologues present only in the subphylum Pezizomycotina of Ascomycota. The recombinant 55-kDa protein of P. marneffei reacted strongly with guinea-pig serum immunized with P. marneffei and with the sera of patients with P. marneffei infection. A similar approach could be applied to immunogenic protein screening in other microorganisms for serological diagnosis, epidemiological studies and the study of vaccines. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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