| Autor: |
Meredith, J. M., Munks, R. J. L., Grail, W., Hurd, H., Eggleston, P., Lehane, M. J. |
| Předmět: |
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| Zdroj: |
Insect Molecular Biology; Aug2006, Vol. 15 Issue 4, p393-401, 9p, 1 Graph |
| Abstrakt: |
A comparative analysis identified key cis-acting regulatory elements responsible for the temporal control of mosquito Defensin gene expression. The promoters of Anopheles gambiae Defensin 1 and two isoforms of Aedes aegypti Defensin A are up-regulated by immune challenge. This stimulated activity depends upon a cluster of three NF-κB binding sites and closely associated C/EBP-like motifs, which function as a unit for optimal promoter activity. Binding of NF-κB and C/EBP like transcription factors is confirmed by electrophoretic mobility shift assay, including supershifts with antibodies to C/EBP. κB-like motifs are abundant within antimicrobial peptide gene promoters and most are very closely associated with putative C/EBP binding sites. This novel association between NF-κB and C/EBP binding sites may, therefore, be of widespread significance. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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