| Autor: |
Young Ha Kim, Joong Hyuk Lee, Nak Whan Paik, Hyune Mo Rho |
| Předmět: |
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| Zdroj: |
DNA & Cell Biology; Jul2006, Vol. 25 Issue 7, p412-417, 6p, 3 Diagrams |
| Abstrakt: |
RNA interference (RNAi) is the process of sequence-specific gene silencing induced by 21–23-nt RNA of small interfering RNA (siRNA). The HBx of hepatitis B virus (HBV) causing human liver diseases has been known as a multifunctional protein which affects transcription, cell growth, and apoptotic cell death. Here, we demonstrate that the HBx-specific siRNA (siRNAx) and short hairpin RNA (shRNAx) effectively induce the degradation of HBx mRNA in HBx-transformed and HBV-producing human liver cells by up to 80–90%. Also, the HBx expression in HBx-transformed cells was continuously silenced by retransformation with the shRNAx expression vector. These results imply that HBx-driven RNAi, either delivery of siRNAx or expression of shRNAx, provides a promising anti-HBV approach to suppress the HBx expression in human hepatoma cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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