Intracellular chemiluminescence activates targeted photodynamic destruction of leukaemic cells.
| Autor: | Laptev, R., Nisnevitch, M., Siboni, G., Malik, Z., Firer, M. A. |
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| Předmět: |
CHEMILUMINESCENCE
PHOTOCHEMOTHERAPY LEUKEMIA TRANSFERRIN BLOOD proteins HEMATOPORPHYRIN BLOOD pigments PORPHYRINS RESEARCH CLINICAL drug trials HETEROCYCLIC compounds PHOTOSENSITIZERS CELL membranes MICROSCOPY TIME RESEARCH methodology CELL physiology ANTINEOPLASTIC agents MEDICAL cooperation EVALUATION research COMPARATIVE studies DOSE-effect relationship in pharmacology CELL lines CYTOPLASM LUMINESCENCE spectroscopy PHARMACODYNAMICS |
| Zdroj: | British Journal of Cancer; 7/17/2006, Vol. 95 Issue 2, p189-196, 8p, 1 Diagram, 1 Chart, 8 Graphs |
| Abstrakt: | Photodynamic therapy (PDT) involves a two-stage process. A light-absorbing photosensitiser (Ps) is endocytosed and then stimulated by light, inducing transfer of energy to a cytoplasmic acceptor molecule and the generation of reactive oxygen species that initiate damage to cellular membrane components and cytolysis. The expanded use of PDT in the clinic is hindered by the lack of Ps target-cell specificity and the limited tissue penetration by external light radiation. This study demonstrates that bioconjugates composed of transferrin and haematoporphyrin (Tf-Hp), significantly improve the specificity and efficiency of PDT for erythroleukemic cells by a factor of almost seven-fold. Fluorescence microscopy showed that the conjugates accumulate in intracellular vesicles whereas free Hp was mostly membrane bound. Experiments with cells deliberately exposed to Tf-Hp at |
| Databáze: | Complementary Index |
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