| Autor: |
Souli, Marie-Christine, Perino, Claude, Piffeteau, Annie, Choquer, Mathias, Malfatti, Pierrette, Cimerman, Agnès, Kunz, Caroline, Boccara, Martine, Vidal-Cros, Anne |
| Předmět: |
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| Zdroj: |
Cellular Microbiology; Aug2006, Vol. 8 Issue 8, p1310-1321, 12p, 8 Color Photographs, 8 Black and White Photographs, 4 Diagrams, 3 Graphs |
| Abstrakt: |
Botrytis cinerea is an important phytopathogenic fungus requiring new methods of control. Chitin biosynthesis, which involves seven classes of chitin synthases, could be an attractive target. A fragment encoding one of the class III enzymes was used to disrupt the corresponding Bcchs3a gene in the B. cinerea genome. The resulting mutant exhibited a 39% reduction in its chitin content and an 89% reduction in its in vitro chitin synthase activity, compared with the wild-type strain. Bcchs3a mutant was not affected in its growth in liquid medium, neither in its production of sclerotia, micro- and macroconidia. In contrast, the mutant Bcchs3a was severely impaired in its growth on solid medium. Counterbalancing this defect in radial growth, Bcchs3a mutant presented a large increase in hyphal ramification, resulting in an enhanced aerial growth. Observations by different techniques of microscopy revealed a thick extracellular matrix around the hyphal tips. Moreover, Bcchs3a mutant had a largely reduced virulence on Vitis vinifera and Arabidopsis thaliana leaves. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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