Evidence for a secretory pathway Ca2+-ATPase in sea urchin spermatozoa

Autor:	Gunaratne, Herath Jayantha, Vacquier, Victor D.
Předmět:	ADENOSINE triphosphatase SEA urchins GERM cells SPERMATOZOA
Zdroj:	FEBS Letters; Jul2006, Vol. 580 Issue 16, p3900-3904, 5p
Abstrakt:	Abstract: Plasma membrane, sarco-endoplasmic reticulum and secretory pathway Ca2+-ATPases (designated PMCA, SERCA and SPCA) regulate intracellular Ca2+ in animal cells. The presence of PMCA, and the absence of SERCA, in sea urchin sperm is known. By using inhibitors of Ca2+-ATPases, we now show the presence of SPCA and Ca2+ store in sea urchin sperm, which refills by SPCA-type pumps. Immunofluorescence shows SPCA localizes to the mitochondrion. Ca2+ measurements reveal that ∼75% of Ca2+ extrusion is by Ca2+ ATPases and 25% by Na+ dependent Ca2+ exchanger/s. Bisphenol, a Ca2+ ATPase inhibitor, completely blocks the acrosome reaction, indicating the importance of Ca2+-ATPases in fertilization. [Copyright &y& Elsevier]
Databáze:	Complementary Index
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