| Autor: |
Fragaki, Konstantina, Kileztky, Claire, Trentesaux, Chantal, Zahm, Jean-Marie, Bajolet, Odile, Johnson, Malcolm, Puchelle, Edith |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Lung Cellular & Molecular Physiology; Jul2006, Vol. 291, pL11-L18, 8p, 1 Color Photograph, 2 Charts, 3 Graphs |
| Abstrakt: |
Although Staphylococcus aureus is a major cause of pulmonary infection, the role played by this bacterium in the induction of inflammation of human airway epithelial cells (HAEC) is poorly understood. In this study, we investigated the inflammatory response of HAEC to S. aureus soluble virulence factors and demonstrate that the combination of a long-acting β2-adrenergic receptor agonist (salmeterol) with a glucocorticoid (fluticasone propionate) has an anti-inflammatory effect on HAEC. First, we demonstrate increased expression at both the mRNA and protein levels of interleukin (IL)-8, IL-6, and tumor necrosis factor (TNF)-α following incubation of HAEC in the presence of S. aureus soluble virulence factors and the increase of 1) the free nuclear factor-κB (NF-κB) and activator protein-I (AP-I) activities and 2) the phosphorylated (P-) extracellular signal-regulated kinases 1 and 2 (ERK1/ERK2), the P-c-Jun NH2-terminal kinase (JNK), and the P-isoform-α of the NF-κB inhibitor (IKBα). Next, when HAEC were preincubated with the combination of salmeterol and fluticasone propionate, the inflammatory response of HAEC was markedly attenuated in that levels of IL-8, IL-6, TNF-α, NF-κB, AP-I, P-ERKI/ERK2, P-JNK, and P-IκBcs decreased significantly. These data emphasize the deleterious effect of S. aureus soluble virulence factors and suggest that the combination of a β2-adrenergic receptor agonist with a glucocorticoid may attenuate the associated airway epithelial inflammation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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