| Autor: |
Petit, Chantal, Rigg, Gordon P., Pazzani, Carlo, Smith, Annabel, Sieberth, Veit, Stevens, Mark, Boulnois, Graham, Jann, Klaus, Roberts, Ian S. |
| Předmět: |
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| Zdroj: |
Molecular Microbiology; Aug1995, Vol. 17 Issue 4, p611-620, 10p, 8 Diagrams, 2 Charts |
| Abstrakt: |
The nucleotide sequence of region 2 of the Escherichia coli K5 capsule gene cluster has been determined. This region, essential for the biosynthesis of the K5 polysaccharide, contained four genes, termed kfiA-D. The G+C ratio was 33.4%, which was lower than the typical G+C ratio for E. coil and that of the flanking regions 1 and 3 in the K5 capsule gene cluster. Three major RNA transcripts were detected within region 2 by Northern blotting and three promoters located by transcript mapping. Promoter activity was confirmed by promoter-probe analysis. The predicted amino acid sequence of KfiC had homology to a number of glycosyl transferase enzymes and overexpression of the kfiC gene resulted in increased K5 transferase activity. The predicted amino acid sequence of KfiD had homology to a number of NAD-dependent dehydrogenase enzymes and was demonstrated to be a UDP-glucose dehydrogenase that catalyses the formation of UDP-glucuronic acid from UDP-glucose. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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