| Autor: |
Yao-Wen Fu, Wei-Gang Wang, Hong-Lan Zhou, Lu Cai |
| Předmět: |
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| Zdroj: |
Asian Journal of Andrology; Jul2006, Vol. 8 Issue 4, p477-482, 6p, 2 Black and White Photographs, 2 Charts, 1 Graph |
| Abstrakt: |
Aim: To examine whether the existence of the donor-and recipient-derived DNA chimerism in recipient's plasma can be a predictive marker for the status of transplanted organ. Methods: One hundred and twenty-six female patients who had been transplanted with male kidneys were enrolled in the present study. In these female recipients, the SRY1, DYZ11st and DYZ12nd genes on the Y chromosome from the plasma were prospectively examined using reverse transcription polymerase chain reaction (RT-PCR). Results: SRY, DYZ11st and DYZ12nd sequences were detected in the cell-free blood (plasma) of 97 (77%) of 126 female patients with male kidney. The average time that the transplanted kidneys functioned was 8.7 years and 5.4 years among microchimerism-positive and microchimerism-negative recipients, respectively. The frequency of the patients who had acute rejection after renal transplantation was approximately 10% and 28% in microchimerism-positive and microchimerism-negative recipients, respectively. Serum creatinine levels in microchimerism-positive patients were significantly lower than those in microchimerism-negative patients. Conclusion: These results suggest that plasma DNA microchimerism present in certain patients following renal transplantation and measurement of plasma DNA microchimerism using quantitative RT-PCR might be a useful predictor for the acceptance of transplanted kidneys. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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