The CII protein of bacteriophage 186 establishes lysogeny by activating a promoter upstream of the lysogenic promoter.

Autor:	Neufing, Petra J., Shearwin, Keith E., Camerotto, Julianne, Egan, J. Barry
Předmět:	BACTERIOPHAGES LYSOGENY PROMOTERS (Genetics) DNA-binding proteins BACTERIAL genetics
Zdroj:	Molecular Microbiology; Aug1996, Vol. 21 Issue 4, p751-761, 11p, 1 Chart, 3 Graphs
Abstrakt:	We have shown previously that the cII gene product of the non-lambdoid temperate bacteriophage 186 is required for the establishment of lysogeny. We show here that CII, a potential helix-turn-helix DNA-binding protein, establishes lysogeny by activating a promoter (pE) which spans the apl/cII intergenic region, upstream of the lysogenic promoter, pL- The start site of the pE transcript (+1) has been mapped by primer extension and we have identified the CII binding determinants at pE by DNase I footprinting. CII binds to inverted repeat sequences separated by two turns of the helix, with binding half-sites centred at the -38 and -58 positions of pE. Oligomerisation studies with purified CII protein indicate that a CII tetramer may be the species that binds to this site. We also show that pE is subject to direct negative feedback by the Cl repressor. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Plný text