Apyrase and 5′-nucleotidase Activities in Synaptosomes from the Cerebral Cortex of Rats Experimentally Demyelinated with Ethidium Bromide and Treated with Interferon-β.

Autor: R. M. Spanevello, C. Mazzanti, R. Kaizer, R. Zanin, D. Cargnelutti, L. Hannel, M. Côrrea, A. Mazzanti, R. Festugatto, D. Graça, M. Schetinger, V. Morsch
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Zdroj: Neurochemical Research; Apr2006, Vol. 31 Issue 4, p455-462, 8p
Abstrakt: Apyrase and 5′-nucleotidase activities were analyzed in an ethidium bromide (EB) demyelinating model associated with interferon-β (IFN-β). The animals were divided in groups: I, control (saline); II, saline and IFN-β; III, EB and IV, EB and IFN-β. After 7, 15 and 30 days the animals (n=5) were sacrificed and the cerebral cortex was removed for synaptosome preparation and enzymatic assays. Apyrase activity using ATP as substrate increased in groups II, III and IV (P<0.001) after 7 days and in groups III and IV (P<0.001) after 15 days. Using ADP as substrate, an activation of this enzyme was observed in group III (P<0.05) after seven and 15 days. The 5′-nucleotidase activity increased in group III (P<0.05) after 7 days and in groups II, III and IV (P<0.001) after 15 days. After 30 days treatment, no significant alteration was observed in enzyme activities. Results showed that apyrase and 5′-nucleotidase activities are altered in demyelination events and that IFN-β was able to regulate the adenine nucleotide hydrolysis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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