| Autor: |
Shimizu, Takao, Ohto, Takayo, Kita, Yoshihiro |
| Předmět: |
|
| Zdroj: |
IUBMB Life; May/Jun2006, Vol. 58 Issue 5/6, p328-333, 6p, 2 Diagrams, 1 Graph |
| Abstrakt: |
Phosphatidylcholine (PC) is a major constituent of biological membranes and a component of serum lipoproteins and pulmonary surfactants. The PC and other glycerophospholipid compositions of membranes change dynamically through stimulus-dependent and independent pathways, principally by the action of two different types of enzymes; phospholipase A 2 [EC 3.1.1.4] and acyl-CoA:lysophospholipid acyltransferase [EC 2.3.1.23]. Phospholipase A 2 is a key enzyme that catalyzes deacylation of the sn-2 position of glycerophospholipids. This enzyme is critical in the remodeling of membrane lipids and formation of two subclasses of lipid mediators, fatty acid derivatives and lysophospholipids. Among many different subtypes of phospholipase A 2 enzymes, we found that cytosolic phospholipase A 2 α (cPLA 2 α) is important in various pathological and physiological responses. Here, we summarize the phenotypes resulting from genetic ablation of cPLA 2 α, and the properties of newly discovered enzymes in the cPLA 2 family. Comprehensive analysis of lipid mediators using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is useful for understanding the roles of individual mediators in physiological and pathological processes. iubmb Life , 58: 328–333, 2006 [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text