Identification of a SCAR marker associated with Bm, the beet mosaic virus resistance gene, on chromosome 1 of sugar beet.

Autor: Friesen, T. L., Weiland, J. J., Aasheim, M. L., Hunger, S., Borchardt, D. C., Lewellen, R. T.
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Zdroj: Plant Breeding; Apr2006, Vol. 125 Issue 2, p167-172, 6p, 1 Black and White Photograph, 2 Diagrams, 3 Charts, 1 Graph
Abstrakt: Beet mosaic virus (BtMV) is an aphid transmitted, viral disease of beet found worldwide. The Bm gene, a resistance gene effective against BtMV, was identified in the sugar beet line 8500 and backcrossed into a C37 background to produce line C719. Three populations were developed from the cross of line C719 with the susceptible line C37 with the intent of developing markers for use in marker-assisted selection. The F2 progeny of three crosses were scored for resistance. Two of the three populations conformed to a 3 : 1 ratio, indicating a single gene trait. Sequence characterized amplified region (SCAR) markers were developed by using bulked segregant analysis combined with random amplified polymorphic DNA type markers. The markers showed close association to the Bm resistance gene and were effective in all three populations. The A 1 allele for genetic male sterility also was found to be associated with Bm and the SCAR marker. Development of a single-nucleotide polymorphism marker from the SCAR sequence was used to validate linkage to chromosome 1 using separate mapping populations. This marker will be useful for the introgression of the Bm gene into germplasm. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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