| Autor: |
Haubitz, M., Wittke, S., Weissinger, E., Rupprecht, H. D., Floege, J., Haller, H., Mischak, H. |
| Předmět: |
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| Zdroj: |
Kidney & Blood Pressure Research; 2004, Vol. 27 Issue 5/6, p408-408, 1/3p |
| Abstrakt: |
Objective: IgA nephropathy (IgA) is the most common chronic glomerular renal disease in adults, leading to hypertension and ultimately to end stage renal disease in about 30 % of the patients. Early intervention and consequent therapy may overcome or at least delay the development of end stage renal disease in these patients. Up to now diagnosis can only be obtained by renal biopsy. Methods: Urine of 43 patients with IgAN was screened for protein/polypeptide patterns with a novel high throughput method, capillary electrophoreses on-line coupled to a mass spectrometer (CE-MS). CE-MS allows fast and accurate evaluation of up to 2000 polypeptides in urine. Results in IgAN were compared to findings in 13 patients with membranous glomerulonephritis (MN) and 57 healthy individuals using support vector machines. Results: A 'normal' polypeptide pattern in urine of healthy volunteers could be established. In patients with IgAN, even when protein excretion was within the normal range of regular tests, the polypeptide pattern in urine differed significantly from those of healthy controls and patients with MN, indicating a specific 'IgAN' pattern. No IgAN patient was classified as normal or as having MN. During follow-up the pattern did not change significantly in patients without immunosuppressive intervention. In patients receiving prednisolone the treatment was followed by changes in the pattern possibly indicating a therapeutic effect. Conclusions: Proteomic analysis with CE-MS coupling permits fast and accurate identification and differentiation of polypeptide patterns in urine of patients with IgAN, allowing differentiation from healthy controls, membranous glomerulonephritis and probably from other renal diseases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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