| Autor: |
Ralph, Steven, Oddy, Claire, Cooper, Dawn, Yueh, Hesther, Jancsik, Sharon, Kolosova, Natalia, Philippe, Ryan N., Aeschliman, Dana, White, Rick, Huber, Dezene, Ritland, Carol E., Benoit, Fran�Xc7;ois, Rigby, Tracey, Nantel, André, Butterfield, Yaron S. N., Kirkpatrick, Robert, Chun, Elizabeth, Liu, Jerry, Palmquist, Diana, Wynhoven, Brian |
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| Zdroj: |
Molecular Ecology; Apr2006, Vol. 15 Issue 5, p1275-1297, 23p, 1 Diagram, 7 Charts, 3 Graphs |
| Abstrakt: |
As part of a genomics strategy to characterize inducible defences against insect herbivory in poplar, we developed a comprehensive suite of functional genomics resources including cDNA libraries, expressed sequence tags (ESTs) and a cDNA microarray platform. These resources are designed to complement the existing poplar genome sequence and poplar ( Populus spp.) ESTs by focusing on herbivore- and elicitor-treated tissues and incorporating normalization methods to capture rare transcripts. From a set of 15 standard, normalized or full-length cDNA libraries, we generated 139 007 3′- or 5′-end sequenced ESTs, representing more than one-third of the c. 385 000 publicly available Populus ESTs. Clustering and assembly of 107 519 3′-end ESTs resulted in 14 451 contigs and 20 560 singletons, altogether representing 35 011 putative unique transcripts, or potentially more than three-quarters of the predicted c. 45 000 genes in the poplar genome. Using this EST resource, we developed a cDNA microarray containing 15 496 unique genes, which was utilized to monitor gene expression in poplar leaves in response to herbivory by forest tent caterpillars ( Malacosoma disstria). After 24 h of feeding, 1191 genes were classified as up-regulated, compared to only 537 down-regulated. Functional classification of this induced gene set revealed genes with roles in plant defence (e.g. endochitinases, Kunitz protease inhibitors), octadecanoid and ethylene signalling (e.g. lipoxygenase, allene oxide synthase, 1-aminocyclopropane-1-carboxylate oxidase), transport (e.g. ABC proteins, calreticulin), secondary metabolism [e.g. polyphenol oxidase, isoflavone reductase, (–)-germacrene D synthase] and transcriptional regulation [e.g. leucine-rich repeat transmembrane kinase, several transcription factor classes (zinc finger C3H type, AP2/EREBP, WRKY, bHLH)]. This study provides the first genome-scale approach to characterize insect-induced defences in a woody perennial providing a solid platform for functional investigation of plant–insect interactions in poplar. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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