Screening of yeasts producing stable L-lactate cytochrome c oxidoreductase and study of the regulation of enzyme synthesis.

Autor: Smutok, O. V., Os'mak, G. S., Gaida, G. Z., Gonchar, M. V.
Předmět:
Zdroj: Microbiology (00262617); Jan2006, Vol. 75 Issue 1, p20-24, 5p, 2 Black and White Photographs, 5 Graphs
Abstrakt: Screening of strains producing a stable form of L-lactate cytochrome c oxidoreductase (flavocytochrome b 2, FC b 2) was carried out among 14 yeast species. Enzyme activity was detected in polyacrylamide gel after the electrophoresis of cell-free extracts. The FC b 2 of Hansenula polymorpha, Rhodotorula pilimanae, and Kluyveromyces lactis are characterized by high thermostability; in particular, the FC b 2 of H. polymorpha retains its activity and tetrameric structure even after heating at 60°C for 10 min. Constitutive synthesis of FC b 2 was observed in H. polymorpha grown on either glucose, ethanol, or glycerol. L-Lactate induces de novo synthesis of FC b 2, as proved by the use of cycloheximide, an inhibitor of protein synthesis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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