| Autor: |
Staquet, M.-J., Couble, M.-L., Roméas, A., Connolly, M., Magloire, H., Hynes, R. O., Clezardin, P., Bleicher, F., Farges, J.-C. |
| Předmět: |
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| Zdroj: |
Cell & Tissue Research; Feb2006, Vol. 323 Issue 3, p457-463, 7p, 1 Diagram, 2 Graphs |
| Abstrakt: |
Integrin αβ heterodimers mediate adhesion to the extracellular matrix and at cell-cell contacts and initiate intracellular signalling cascades in response to a variety of inductive factors. Apart from the expression of αvβ3 that we have previously reported, little is known about the expression of integrins in odontoblasts. Here, we investigated the expression of αv-binding β integrin subunits in healthy human dental pulp in vivo and in odontoblasts differentiated in vitro. Reverse transcription/polymerase chain reaction analysis revealed the expression of αv, β1, β5 and β8 integrin mRNA, but not β6, in whole pulp cells. Flow cytometry showed that the αv and β1 subunits were the most intensely expressed. Immunohistochemistry demonstrated that the β1 subunit was localised in newly differentiated odontoblasts in the root and in mature odontoblasts in the crown, including their intradentinal cell processes. The αv chain was predominantly expressed by mature odontoblasts and αvβ5 was only observed in mature odontoblasts. In vitro differentiated odontoblasts expressed genes for αv, β1 and β5, but not for β6 and β8. A comparison of integrin profiles between cultured pulp cells and in vitro differentiated odontoblasts revealed that odontoblast maturation was characterised by a significant increase in the expression of αv and β1 subunits and αvβ5 integrin. The β8 subunit was detected in nerve cells only. Histological analysis of teeth from αv knockout mice showed no obvious structural modification in the odontoblast layer. Thus, human mature odontoblasts express αvβ3, αvβ5 and perhaps αvβ1 integrins, with the possible presence of α-β1 pairs. The roles that these molecules play in the exchange of information throughout the odontoblast layer remain to be determined. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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