| Autor: |
Chen, X.-B., Regan, J. W. |
| Předmět: |
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| Zdroj: |
Cellular & Molecular Life Sciences; Jan2006, Vol. 63 Issue 1, p112-121, 10p, 1 Color Photograph, 6 Graphs |
| Abstrakt: |
Studies have shown prostaglandin F2α $$\left( {{\text{PGF}}_{2\alpha } } \right)$$ to be an endogenous tumor promoter in mouse models of skin carcinogenesis; however, the mechanisms by which PGF2α affects cell cycle events remain unknown. Here we performed cell cycle analyses on HEK cells stably expressing the human FP receptor and found that treatment with PGF2α delays mitosis and is associated with an increased expression of cyclin B1 and Cdc2 kinase activity. In addition, multipolar spindles and misaligned chromosomes were observed in a significant proportion of cells treated with PGF2α. Defective cytokinesis was also observed which resulted in gross aneuploidy and polyploidy. Expression of dominant negative Rho attenuated the cell cycle delay and prevented the generation of micronuclei following treatment with PGF2α. This suggests that FP receptor activation of Rho signaling by PGF2α can interfere with nuclear division. Aneuploidy is associated with genomic instability and may underlie the tumor-promoting properties of PGF2α. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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