Generation of mouse-human hybridomas secreting human monoclonal antibodies to Japanese cedar pollen allergen Cry j1.

Autor: Naganawa, Yasunori, Shinmoto, Hiroshi, Shimmoto, Michie
Předmět:
Zdroj: Human Antibodies; 2005, Vol. 14 Issue 1/2, p27-31, 5p, 2 Charts, 1 Graph
Abstrakt: Peripheral blood lymphocytes from 13 donors were transformed with Epstein-Barr virus (EBV) to establish immortalized human B-cells secreting antibodies to Japanese cedar (Cryptomeria japonica) pollen allergens. EBV-transformed B-lymphocytes were then fused with mouse myeloma SP2/O3, and three clones of mouse-human hybridomas secreting human IgM class monoclonal antibodies to a cedar pollen allergen Cry j1 were established. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index