CD14+ cell collection in non-cytokine-stimulated donors with the COM.TEC cell separator.

Autor: Strasser, Erwin F., Hendelmeier, Martin, Weisbach, Volker, Zimmermann, Robert, Ringwald, Jürgen, Juntke, Ronald, Sauer, Gudrun, Zingsem, Jürgen, Eckstein, Reinhold
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Zdroj: Transfusion; Jan2006, Vol. 46 Issue 1, p66-73, 8p, 2 Diagrams, 5 Charts
Abstrakt: BACKGROUND: The COM.TEC cell separator (Fresenius Hemocare), equipped with the MNC program (single-stage chamber) and the PBSC-LYM program (dual-stage chamber), was evaluated for CD14+ cell collection regarding cell yields, collection efficiencies (CEs), and the content of residual cells in harvests. STUDY DESIGN AND METHODS: Twenty-four non-cytokine-stimulated donors underwent 5-L mononuclear cell (MNC) collections on the COM.TEC device to compare both programs. Two software versions (v02.03.05 vs. v03.00.04) were investigated for optimization of the CD14+ cell collection process. Blood counts of donors and products were analyzed for CD14+ cells by flow cytometry and for platelets (PLTs), white blood cells, and red blood cells (RBCs) by a blood cell counter. RESULTS: In 5-L collections, the MNC program resulted in high CEs (83 ± 23%) and yields (1.2 × 109 ± 0.6 × 109 per unit) of CD14+ cells, but the products showed high residual PLTs. The use of a dual-stage chamber in the PBSC-LYM program produced a low content of residual PLTs (0.7 × 1011 ± 0.3 × 1011 per unit) and RBCs but failed to reach a target of 1 × 109 CD14+ cells. Modulated light to stabilize the buffy-coat detection by the interface monitor significantly improved CD14+ cell enrichment. By use of the PBSC-LYM program, higher centrifuge velocity (1700 rpm [382 × g] vs. 1500 rpm [297 × g]) improved significantly CD14+ cell yields (0.7 × 109 vs. 0.5 × 109 cells). CONCLUSION: A pure CD14+ cell product with low numbers of residual cells was obtained by the PBSC-LYM program, which could be useful for good manufacturing practice–conformed production within closed systems. The MNC program offers the collection of high CD14+ cell yields with excellent CEs but also high residual PLT counts. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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