Application of two SH-based methods for metallothionein determination in mussels and intercalibration of the spectrophotometric method: laboratory and field studies in the Mediterranean Sea.

Autor:	Zorita, I., Strogyloudi, E., Buxens, A., Mazón, L. I., Papathanassiou, E., Soto, M., Cajaraville, M. P.
Předmět:	METALLOTHIONEIN POLAROGRAPHY SPECTROMETRY METALLOPROTEINS ORGANOMETALLIC compounds ORGANOSULFUR compounds ELECTROCHEMICAL analysis
Zdroj:	Biomarkers; Sep/Oct2005, Vol. 10 Issue 5, p342-359, 18p, 1 Diagram, 3 Charts, 8 Graphs
Abstrakt:	Metallothionein (MT) induction is widely used as a biomarker of exposure to metals in mussels. The aims of the present work were first to compare the suitability of spectrophotometry and differential pulse polarography (DPP) for MT detection in mussels exposed to 200 ppb cadmium for 9 days in a laboratory experiment and in mussels sampled in different seasons from expected pollution gradients along the Mediterranean Sea; second, to intercalibrate the widely used spectrophotometric method using mussels from Saronikos Gulf. In the intercalibration of the spectrophotometric method, similar results ( p >0.05) were obtained by two different research teams indicating a good reproducibility of the technique. However, polarographic and spectrophotometric methods gave significantly ( p [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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