| Autor: |
Kyo Jeoung, Min, Bok Chung, Youn, Sok Lee, Pung, Soo Kim, Chang, Hee Kim, Nam, Ju Son, Dong, Tae Hong, Jin, Moon, Dong‐Cheul |
| Předmět: |
|
| Zdroj: |
Journal of Liquid Chromatography & Related Technologies; 2005, Vol. 28 Issue 20, p3205-3216, 12p, 1 Diagram, 3 Charts, 3 Graphs |
| Abstrakt: |
We developed a liquid chromatography‐tandem mass spectrometric (LC‐MS/MS) method for the determination of a ginseng saponin metabolite, IH901 or compound K (20‐O‐β‐D‐glucopyranosyl‐20(S)‐protopanaxadiol) in rat plasma. The method involves protein precipitation by acetonitrile and HPLC separation of the sample extracts on a reversed‐phase column (X‐terra™ RP18) with isocratic elution of 20 mM ammonium acetate and acetonitrile (30:70, v/v), at a flow rate of 0.2 mL/min. The MS analysis was performed by electrospray positive ionization mass spectrometry using multiple reaction‐monitoring mode. The mass transitions of IH901 and prednisolone (internal standard) were m/z 640→425 and 361→343, respectively. The standard curves for IH901 were linear over the concentration range of 2.0–500 ng/mL. The lower limit of quantification was 2 ng/mL and the limited of detection was 1 ng/mL for IH901. The coefficient of variation of intra‐ and inter‐day assays at five quality control levels ranged from 2.0 to 13.9% and the accuracy varied between 93.2 and 110.5%. This method was used to determine IH901 in plasma samples after the oral administration of a single 30 mg/kg dose on SD rats. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
|
Nepřihlášeným uživatelům se plný text nezobrazuje |
K zobrazení výsledku je třeba se přihlásit.
|
