| Autor: |
Serrani, Federica, Berardi, Enrico |
| Předmět: |
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| Zdroj: |
FEMS Yeast Research; Nov2005 Special Issue, Vol. 5, p999-1007, 9p |
| Abstrakt: |
Abstract: To establish a basis for genetic and molecular studies of nitrite assimilation in the methylotrophic yeast Hansenula polymorpha, we isolated and characterised six nitrite-negative mutants still capable of growing on nitrate. Gene isolation work yielded the NII2 gene, encoding a membrane protein homologous to the Saccharomyces cerevisiae Pho86p. Sequence analysis revealed an ORF of 860 bp encoding a 286-amino-acid protein with a predicted molecular mass of 32.8kDa. This protein is shorter than its S. cerevisiae homologue, and is predicted to lack an ER-retention signal. Cell suspension work revealed that the null mutant is unable to take up nitrite from the medium. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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