| Autor: |
Pon, R. T., Yu, S., Prabhavalkar, T., Mishra, T., Kulkarni, B., Sanghvi, Y. S. |
| Předmět: |
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| Zdroj: |
Nucleosides, Nucleotides & Nucleic Acids; May2005, Vol. 24 Issue 5-7, p777-781, 5p, 3 Diagrams |
| Abstrakt: |
Small interfering RNAs (siRNA) are the latest candidates for oligonucleotide-based therapeutics. Should siRNA be successful in clinical trials, a huge demand for synthetic RNA is anticipated. We believe that 1-(4-chlorophenyl)-4-ethoxypiperidin-4-yl (Cpep) is an ideal 2′-protecting group for large-scale syntheses. Unlike 2′-silyl groups, mild acid hydrolysis instead of fluoride ion is used for the 2′-deprotection. The syntheses of 2′-Cpep protected nucleosides (A, C, G, and U) has been accomplished on a 0.5 Kg scale. The 2′-Cpep monomers were transformed into 3′- O -phosphoramidites for conventional automated solid-phase synthesis. Cost-effective processes for large-scale synthesis of Cpep monomers and initial automated solid-phase synthesis are demonstrated. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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