Oxidation of Biotin During Oligonucleotide Synthesis.

Autor: Upadhya, Krishna, Khattak, Iqbal K., Mullah, Bashar
Předmět:
Zdroj: Nucleosides, Nucleotides & Nucleic Acids; May2005, Vol. 24 Issue 5-7, p919-922, 4p, 1 Diagram, 1 Chart, 1 Graph
Abstrakt: A new “polystyrene biotin support” has been synthesized for the solid support synthesis of the 3′-biotinylated oligonucleotides. Several oligos were synthesized and were analyzed by the HPLC and Mass Spec. Oligo analysis revealed that the biotin gets oxidized to “biotin sulfoxide” during the synthesis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index