| Autor: |
Bîrsan, Tudor, Dambrin, Camille, Freitag, Derrick G., Yatscoff, Randall W., Morris, Randall E. |
| Předmět: |
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| Zdroj: |
Transplant International; Dec2004, Vol. 17 Issue 12, p767-771, 5p |
| Abstrakt: |
ISA247 is a novel cyclosporine analog. In this study we compare, in vitro, the effects of ISA247 on immune function with those of cyclosporine. Whole blood from cynomolgus monkeys. (n =5) was incubated with different concentrations of ISA247 or cyclosporme and stimulated with different mitogens in culture medium, Lymphocyte proliferation was assessed by [³H]-TdR incorporation assay and by flow cytometry. Flow cytometry was also used to assess production of intracellular cytokines by T cells and expression of T cell activation surface antigens. The concentration of drug necessary to attain 50% of the maximum effect (EC50) was subsequently calculated. EC50 values for ISA247 were lower than for cyclosporine, and the differences were statistically significant for lymphocyte proliferation, T cell cytokine production, and expression of all T cell activation surface antigens but one. We conclude that ISA247 suppresses diverse immune functions more potently than cyclosporine in vitro. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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