| Autor: |
Fujita, Yukihiro, Yamaguchi, Kosaku, Kamegaya, Taichi, Sato, Hiroko, Semura, Kayoko, Mutoh, Kenichiro, Kashimoto, Takashige, Ohori, Hitoshi, Mukai, Takao |
| Předmět: |
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| Zdroj: |
Helicobacter; Dec2005, Vol. 10 Issue 6, p567-576, 10p, 2 Black and White Photographs, 2 Charts, 4 Graphs |
| Abstrakt: |
Background. Helicobacter pylori survival in a hostile acidic environment is known to be caused by its production of urease, which is not released by known secretion pathways. It has been proposed that H. pylori cells undergo spontaneous autolysis during cultivation and that urease becomes surface-associated only concomitant with bacterial autolysis. The aim of this study was to elucidate mechanisms by which H. pylori cells undergo autolysis during cultivation. Materials and Methods. Autolysis of H. pylori KZ109 cells was estimated by measuring the turbidity of the culture, by detection of cytoplasmic protein release into the culture supernatant and by scanning electron microscopic observation of H. pylori cells during cultivation. An autolysis-inducing factor (AIF) was partially purified from the culture supernatant by a partition method using ethyl acetate. Results. Bacterial turbidity of KZ109 cells was drastically decreased after late-log phase accompanying release of urease and HspB into the extracellular space. Concomitantly, cell lytic activity was detected in the culture supernatant. Scanning electron microscopic observation suggested that partially purified AIF induced cell lysis. It was also shown that the AIF is different from other autolytic enzymes or substances so far reported. Conclusions. This study demonstrated the presence of the peptidergic autolytic substances in the culture supernatant of H. pylori KZ109 cells. The results of this study should be useful for further studies aimed at elucidation of the strategy of survival of H. pylori in the gastric environment and elucidation of the mechanisms of pathogenesis induced by H. pylori. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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