| Autor: |
Valenzuela-Avendaño, Juan P., Estrada Mota, Iván A., Uc, Gabriel Lizama, Souza Perera, Ramón, Valenzuela-Soto, Elisa M., Zúñiga Aguilar, José J. |
| Předmět: |
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| Zdroj: |
Plant Molecular Biology Reporter; Jun2005, Vol. 23 Issue 2, p199-200, 2p |
| Abstrakt: |
Isolation of high-quality RNA is a necessary step in gene expression analysis. Although many methods can be used to isolate RNA from plants where contamination of preparations with complex carbohydrates or phenolic compounds is a problem, the application of these methods to Selaginella lepidophylla tissues has failed to obtain goodquality RNA. Here we introduce 2 modifications to the method developed by Chomczynski and Sacchi (1987), generating a simple and rapid method that allows the isolation of intact RNA from S. lepidophylla-dshydrated tissues. Although the introduced modifications are not new, their addition proved to be decisive for success in RNA isolation. Quality of the RNA obtained was evaluated by electrophoresis in agarose and by 3 different PCR-based techniques--RT-PCR, RNA differential display, and synthesis of a cDNA library. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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