| Autor: |
Woo, Patrick C.Y., Ma, Shirley S.L., Teng, Jade L.L., Li, Maria W.S., Kao, Richard Y.T., Lau, Susanna K.P., Yuen, Kwok-yung |
| Předmět: |
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| Zdroj: |
FEMS Microbiology Letters; Nov2005, Vol. 252 Issue 1, p57-65, 9p |
| Abstrakt: |
Abstract: An Escherichia coli–Laribacter hongkongensis shuttle vector (pPW380) was constructed by ligating the 4701-bp EcoRI digested fragment of pHLHK8 to EcoRI digested pBK-CMV. An E. coli–L. hongkongensis inducible expression shuttle vector was further constructed by ligating a 2105-bp fragment that contains the tetracycline repressor and tetracycline-inducible promoter region of pALC2084 to the 8897-bp fragment of pPW380, deletion of the green fluorescent protein gene, and insertion of a multiple cloning site. This inducible expression system was able to express two commonly used reporter genes, the green fluorescent protein gene and the glutathione S-transferase gene, efficiently in E. coli and L. hongkongensis. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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Plný text