| Autor: |
Griggi, T., Bauer, R., Garofalo, T., Kukel, S., Lenti, L., Massetti, A. P., Müller, C., Sorice, M., Pontieri, G. M. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Jul1994, Vol. 40 Issue 1, p77-82, 6p, 3 Charts, 2 Graphs |
| Abstrakt: |
In this study we analysed the relationship between anti-lymphocytic ganglioside antibodies and anti-lymphocyte antibodies in AIDS patients. Anti-lymphocytic ganglioside antibodies were detected by thin layer chromatography (TLC) immunostaining; three colour flow cytometry was used to analyse circulating antibodies against different lymphocyte subsets. Anti-lymphocytic ganglioside antibodies were detected in 23 out of 49 AIDS patient sera (46.9%). All positive sera reacted selectively with the GM3 comigrating band from AIDS lymphocytes. Twenty two out of the 23 anti-lymphocytic GM3 positive sera also had antibodies against CD4+ T cells, versus 17/26 anti-GM3 negative. Furthermore, patients with lymphocytic GM3 antibodies showed a significantly higher antibody reactivity against CD4+ T cells than patients in which these antibodies were not detected. The absorption tests revealed that preincubation of positive sera with GM3 was followed by a decrease in the reaction with target lymphocytes. These findings suggest that anti-GM3 antibodies are a portion, but not the majority, of antibodies reacting with CD4+ T cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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