| Autor: |
Dutra, W. O., Gollob, K. J., Pinto-Dias, J. C., Gazzinelli, G., Correa-Oliveira, R., Coffman, R. L., Carvalho-Parra, J. F. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Jan1997, Vol. 45 Issue 1, p74-80, 7p, 1 Chart, 6 Graphs |
| Abstrakt: |
Characterization of immunologic activities during chronic infection with Trypanosoma cruzi is critical for understanding the dynamics of human Chagas' disease. Since cytokine production is mainly regulated by transcription and mRNA stability, quantitative RT-PCR analysis gives an accurate picture of the influences of disease on cytokine profile. Using RT-PCR. the authors analysed the levels of message expression for several cytokines in peripheral blood mononuclear cells (PBMC) freshly isolated from chagasic patients (CP) and non-infected individuals (NI), and in in vitro-stimulated PBMC from CP. Ex vivo analysis showed that mean levels of expression of IL-5, IL-10, IL-13 and IFNγ were dramatically increased in PBMC from CP, compared to NI. The levels of IL-2 and IL-4 were not significantly different between groups. Analysis of cytokine mRNA production after in vitro culture with parasite-derived antigens (EPI or TRP) or anti-epimastigote antibodies (Id) showed that these two classes of stimuli induced distinct cytokine responses. While EPI or TRP induced higher production of IFNγ specific message and low IL-10, anti-Id cells produced higher levels of IL-10 and low IFNγ. The simultaneous presence of antigenic and antibody stimulation in the host during the chronic phase of Chagas' disease could explain the existence of both inflammatory and anti-inflammatory cellular reactivity detected in most patients. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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