| Autor: |
Williams, S. A., Vik, D. P. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Jan1997, Vol. 45 Issue 1, p7-15, 9p, 3 Diagrams, 3 Graphs |
| Abstrakt: |
The promoter region of the human factor H gene was cloned and a 3 kb Eco RI fragment was sequenced. Primer extension and S1 nuclease analysis were used to determine the transcription start site, which was found to be 10-11 nucleotides upstream of the published cDNA sequence. No canonical TATA or CCAAT boxes were found in conjunction with this site. The sequence from the human H promoter region was compared to that from the mouse gene. There was a region of 800 bp that was 62.5% identical between the two sequences. The sequences of the two promoter regions were compared to a database of transcription factor binding sites. Five elements were identified that matched the consensus sequence 100% and were identical in the two promoter sequences. Promoter assays using the luciferase reporter gene demonstrated that this region contained a functional transcription start site and putative enhancer elements. U118-MG astroglioma cells and Hep3b hepatoma cells were incubated with various cytokines to measure effects on their factor H mRNA levels. Interferon-γ (IFN-γ). but not interleukin-1 (IL-1), tumour necrosis factor α (TNF-α) or IL-6, was able to increase the level of H mRNA in both cell lines. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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