Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds.

Autor: de Almeida Gadelha, Carlos Alberto, Moreno, Frederico Bruno Mendes Batista, Santi-Gadelha, Tatiane, Cajazeiras, João Batista, da Rocha, Bruno Anderson M., Rustiguel, Joane Kathelen Rodrigues, Freitas, Beatriz Tupinamba, Canduri, Fernanda, Delatorre, Plínio, de Azevedo Jr., Walter Filgueira, Cavada, Benildo S.
Předmět:
Zdroj: Acta Crystallographica: Section F (Wiley-Blackwell); Jan2005, Vol. 61 Issue 1, p87-89, 3p
Abstrakt: A lectin from Canavalia maritima seeds (ConM) was purified and submitted to crystallization experiments. The best crystals were obtained using the vapour- diffusion method at a constant temperature of 293 K and grew in 7 d. A complete structural data set was collected to 2.1 Å resolution using a synchrotron-radiation source. The ConM crystal belongs to the orthorhombic space group P21212, with unit-cell parameters a = 67.15, b = 70.90, c = 97.37 Å. A molecular-replacement search found a solution with a correlation coefficient of 69.2% and an R factor of 42.5%. Crystallographic refinement is under way. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index