| Autor: |
Nina Abou Ghanem-Sabanadzovic, Sead Sabanadzovic, Michele Digiaro, Giovanni P. Martelli |
| Předmět: |
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| Zdroj: |
Virus Genes; May2005, Vol. 30 Issue 3, p335-340, 6p |
| Abstrakt: |
Abstract The nucleotide sequence of RNA-2 of Grapevine Anatolian ringspot virus (GARSV) and Grapevine deformation virus (GDefV), two recently described nepoviruses, has been determined. These RNAs are 3753 nt (GDefV) and 4607 nt (GARSV) in size and contain a single open reading frame encoding a polyprotein of 122 kDa (GDefV) and 150kDa (GARSV). Full-length nucleotide sequence comparison disclosed 7173% homology between GDefV RNA-2 and that of Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV), and 6264% homology between GARSV RNA-2 and that of Grapevine chrome mosaic virus (GCMV) and Tomato black ring virus (TBRV). As previously observed in other nepoviruses, the 5' non-coding regions of both RNAs are capable of forming stem-loop structures. Phylogenetic analysis of the three proteins encoded by RNA-2 (i.e. protein 2A, movement protein and coat protein) confirmed that GDefV and GARSV are distinct viruses which can be assigned as definitive species in subgroup A and subgroup B of the genus Nepovirus, respectively. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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