Low-level arsenite activates the transcription of genes involved in adipose differentiation.

Autor: B. Salazard, L. Bellon, S. Jean, M. Maraninchi, C. El-Yazidi, T. Orsire, A. Margotat, A. Botta, J.-L. Berg-Lefranc
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Zdroj: Cell Biology & Toxicology; Nov2004, Vol. 20 Issue 6, p375-385, 11p
Abstrakt: Abstract In this study we analyzed gene expression in 3T3-F442A pre-adipocyte cells that differentiate in the presence of micro-molar arsenate concentration. Two concentrations of arsenite (As2O3, 0.25 mol/L and 0.5 mol/L) were applied for three days with and without insulin (170 nmol/L) and gene expressions were evaluated by quantitative RT-PCR. The genes included genes of oxidative-stress responses: heme-oxygenase-1 (HO1) and the hypoxia inducible factor 1a (HIF1a), genes of cell-cycle: c-jun and Kruppel like factor 5 (KLF5), and genes that play important roles in adipose determination: a peroxisome proliferator-activated receptor (PPAR?) and a CCAAT/ enhancer binding protein (C/EBPa). Arsenite induced the expression of HO1, HIF1a, KLF5, PPAR? and C/EBPa. These results suggest that under condition of oxidative stress arsenite induces genes that are required for adipose differentiation. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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