| Autor: |
T. Kamei, N. M. Toriello, E. T. Lagally, R. G. Blazej, J. R. Scherer, R. A. Street, R. A. Mathies |
| Předmět: |
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| Zdroj: |
Biomedical Microdevices; Jun2005, Vol. 7 Issue 2, p147-152, 6p |
| Abstrakt: |
Abstract We have developed an integrated hydrogenated amorphous silicon (a-Si:H) fluorescence detector for microfluidic genetic analysis. It consists of a half-ball lens, a ZnS/YF3 multilayer optical interference filter with a pinhole, and an annular a-Si:H PIN photodiode allowing the laser excitation to pass up through the central aperture in the photodiode and the filter. Microfluidic separations of multiplex PCR products generated from methicillin-resistant/sensitive Staphylococcus aureus (MRSA/MSSA) DNA on microfluidic capillary electrophoresis (CE) devices are successfully detected with the integrated detector. Similarly, multiplex PCR amplicons from the kanamycin resistant and K12 serotype-specific genes of E. coli cells are detected. The direct detection of multiplex PCR amplicons indicates that the fluorescence detector can be successfully coupled with current microfluidic PCR-CE platforms. This work establishes that the integrated a-Si:H detector provides relevant limits of detection for point-of-care genetic and pathogen analysis with microfluidic devices. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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