| Autor: |
Monika Jain, Rakha Hari Das |
| Předmět: |
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| Zdroj: |
Molecular Biology Reports; Dec2004, Vol. 31 Issue 4, p261-266, 6p |
| Abstrakt: |
Abstract An ORF having a potential to code for a polypeptide of 79 amino acids has been identified within 993 nt sequence of 2 kb Eco RI-W fragment of Spodoptera litura nucleopolyhedrovirus (SpltNPV-I). Nucleotide and deduced amino acid sequence analyses showed its identity with the ubiquitin homologue of eukaryotes (7980%), Melanoplus sanguinipes entomopoxvirus (76%) and other baculoviruses (7289%). The ORF is under baculovirus late promoter motif RTAAG but unlike other baculoviruses, three such motifs at -6, -10 and -27 position are present in SpltNPV. The ORF expresses as a 10 kDa protein in E. coli and the purified recombinant protein showed crossreactivity with the rabbit anti-ubiquitin antibodies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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