| Autor: |
Kunnavatana, Shaun S., Quan, Susan Y., Koch, R. James |
| Předmět: |
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| Zdroj: |
Archives of Otolaryngology - Head & Neck Surgery; Sep2005, Vol. 131 Issue 9, p809-814, 6p |
| Abstrakt: |
Objective To examine the combined effect of hyperbaric oxygen and N-acetylcysteine, a well-studied antioxidant, on fibroblast proliferation and production of 3 specific growth factors: basic fibroblast growth factor, vascular endothelial growth factor, and transforming growth factor β1. Design In vitro study. Subjects None. Interventions Human dermal fibroblasts were propagated in serum-free medium and subjected to daily 90-minute 2-atm hyperbaric oxygen treatments with varying concentrations of N-acetylcysteine for 7 consecutive days. Cell proliferation and growth factor assays were performed on days 0, 1, 3, 5, and 7. Results Population doubling time decreased significantly with 40 μmol/L of N-acetylcysteine supplementation of 2-atm hyperbaric oxygen treatment. Higher levels of N-acetylcysteine increased population doubling time. Conclusions Supplementation of hyperbaric oxygen therapy with 40 μmol/L of N-acetylcysteine appears to increase fibroblast proliferation without producing an unfavorable growth factor profile for normal healing. This suggests that this level of N-acetylcysteine may foster an ideal redox environment for fibroblast proliferation in a hyperbaric oxygen environment. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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