| Abstrakt: |
This article presents information on a study on the detection of simple sequence length polymorphisms (SSLP). Analysis of SSLP, or microsatellites, is a tool for paternity determinations and population screening. However, because it is desirable to avoid radioactive compounds, the researchers tested silver staining of the polyacrylamide gels as an alternative in detecting SSLP. They also showed that the required two nucleotide resolution for the analysis of SSLP can be obtained on standard gel sizes by comparing silver staining and radioactive detection of the same SSLP loci. However, the researchers also found that the silver stained gels tend to show more artefact bands, which may be due to heteroduplex formation. Moreover, the most convenient markers on standard sequencing gels are normal sequencing reactions, which cannot be used on non-denaturing gels. |
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