Gliclazide Attenuates the Intracellular Ca 2+ Changes Induced In Vitro by Ischemia in the Retinal Slices of Rats with Streptozotocin-Induced Diabetes.

Autor: Kinukawa, Jiro, Shimura, Masahiko, Harata, Nobutoshi, Tamai, Makoto
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Zdroj: Current Eye Research; Sep2005, Vol. 30 Issue 9, p789-798, 10p
Abstrakt: Purpose : To investigate the dynamics of the intracellular Ca 2+ concentration ([Ca 2+ ] i ) during retinal ischemia in rats with streptozotocin (STZ)-induced diabetes and the effect of gliclazide, a sulfonylurea with a potent free-radical scavenging activity on ischemia-induced [Ca 2+ ] i dynamics. Methods : Rats with STZ (65 mg/kg) induced diabetes were divided into three groups: the untreated diabetic group, the gliclazide-treated group, and the glibenclamide-treated group. An ischemic condition was imposed in vitro on the retinal slices by perfusion with an oxygen/glucose deprived solution. The [Ca 2+ ] i was measured in individual layers of the rat retinal slices loaded with the Ca 2+ indicator fluo-3. Results : As compared to that in the normal rat retina, both the amplitude and the kinetics of the [Ca 2+ ] i increase were suppressed in the intermediate layers of the retinal slices from the diabetic rats under the ischemic condition. These changes were attenuated by the administration of gliclazide but not by that of glibenclamide. Conclusions : Hyperglycemia influences ischemia-induced [Ca 2+ ] i dynamics predominantly in the intermediate layers of the retina, and gliclazide, as compared to glibenclamide without a free radical scavenging activity, potently attenuates the ischemia-induced changes in the calcium dynamics. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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