Cloning and nucleotide sequence analysis of the serotype 2 fimbrial subunit gene of Bordetella pertussis.

Autor: Livey, I., Duggleby, C. J., Robinson, A.
Předmět:
Zdroj: Molecular Microbiology; Sep1987, Vol. 1 Issue 2, p203-209, 7p, 3 Graphs
Abstrakt: An oligonucleotide probe complementary to the beginning of the gene encoding the serotype 2 (ST2) fimbrial subunit of Bordetella pertussis was synthesized and a cloned DNA fragment hybridizing with the probe identified and sequenced. Several lines of evidence indicate that an open reading frame with coding Information for a polypeptide of 207 amino acids, including a 26-amino-acid signal sequence, is the ST2 gene. The protein deduced from the nucleotide sequence shows good agreement with the NH2-terminal amino acid sequence, amino acid composition and molecular weight of the purified fimbrial subunit. In addition, the proposed ST2 subunit is shown to have homology with other fimbrial subunits. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index