Autor: |
Dobryan M. Tracz, David A. Boyd, Louis Bryden, Romeo Hizon, Sandra Giercke, Paul Van Caeseele, Michael R. Mulvey |
Předmět: |
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Zdroj: |
Journal of Antimicrobial Chemotherapy (JAC); May2005, Vol. 55 Issue 5, p768-772, 5p |
Abstrakt: |
Objectives: To characterize the mechanism of cefoxitin resistance in clinical isolate Escherichia coli N99-0001.Methods: Plasmid analysis, PCR for -lactamases, and sequencing of the ampC genes was carried out. An RTPCR method was developed to determine relative ampC expression.Results: Analysis of the ampC promoter region of E. coli N99-0001 revealed a T?A mutation at -32, a C?A mutation at -11, an insertion of a T between -20 and -21, and a 28?bp deletion including the entire attenuator. RTPCR showed that ampC was expressed 140-fold higher in E. coli N99-0001 than in E. coli ATCC 25922.Conclusions: Cefoxitin resistance in E. coli N99-0001 was due to overexpression of ampC caused by an increase in promoter strength. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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