| Autor: |
Luiz R. Travassos, Luis S. Silva, Elaine G. Rodrigues, Stefania Conti, Antonella Salati, Walter Magliani, Luciano Polonelli |
| Předmět: |
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| Zdroj: |
Journal of Antimicrobial Chemotherapy (JAC); Nov2004, Vol. 54 Issue 5, p956-958, 3p |
| Abstrakt: |
Objectives: To evaluate whether an engineered synthetic decapeptide (KP) derived from the sequence of a recombinant anti-idiotypic antibody, that represents the internal image of a Pichia anomala killer toxin, could be fungicidal in vitro and therapeutic in vivo against Paracoccidioides brasiliensis and paracoccidioidomycosis (PCM).Methods: Fungicidal activity of KP was assessed in vitro and in vivo by inhibition of colony forming units and by histological examination, 8 days after infection, of organs from mice intravenously injected with a virulent strain of P. brasiliensis (3??106?yeast cells) and intraperitoneally treated with KP (3.3?g/g body weight, three doses), in comparison with control animals equally administered with a scrambled decapeptide (SP).Results: KP but not SP was fungicidal in vitro at 39?ng/multiply-budding yeast cell and less efficiently in its D-isomeric form (0.31?g/multiply-budding yeast cell). It was also able to markedly reduce the fungal load in organs (liver, lung, spleen) of infected animals.Conclusions: The therapeutic effect observed opens the way for using the antifungal peptide as an alternative control of PCM in association with conventional antifungal drugs. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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