Impacts of Black Soldier Fly (Hermetia illucens) Larval Meal on Intestinal Histopathology and Microbiome Responses in Hybrid Grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂): A Comprehensive Analysis.

Autor: Chen, Yan, Ma, Jun, Yong, Yoong-Soon, Chen, Yonggan, Chen, Bing, Cao, Junming, Peng, Kai, Wang, Guaxia, Huang, Hai, Loh, Jiun-Yan
Předmět:
Zdroj: Animals (2076-2615); Dec2024, Vol. 14 Issue 24, p3596, 24p
Abstrakt: Simple Summary: Fishmeal is commonly used in aquafeed manufacturing. However, the global market price of fishmeal is highly fluctuating, and it is not the most sustainable solution. Therefore, the aquafeed industry is often exploring alternative protein sources such as insect meal to replace fishmeal. Our study involved testing diets where 10%, 30%, and 50% of the fishmeal was replaced with black soldier fly larvae to observe how this affects digestion and gut bacteria diversity in hybrid grouper. The dietary group with 10% substitution showed higher levels of enzymes that help break down food and absorb nutrients effectively. In contrast, the diets with 30% and 50% replacements showed signs of weakening in the intestinal walls, which could negatively affect nutrient absorption. Moreover, the variety of gut bacteria in the groups with 10% and 50% replacement was greater, with modifications observed in the biological functions associated with energy and nutrient processing. Our results suggest that replacing some fishmeal with black soldier fly larval meal could potentially improve gut health and promote the development of sustainable aquaculture diets. This study examined the diversity and responses of intestinal microbiota in hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) fed diets with varying levels of fishmeal replaced by black soldier fly larvae (BSFL). The 10% BSFL substitution (BSFL10) group showed the highest levels of trypsin and amylase. Substituting fishmeal with 30% and 50% BSFL weakened the intestinal wall, resulting in vacuoles, sparse striatal boundaries, and fewer villi. Microbiota diversity, measured through Shannon's index, was higher in the BSFL10 and BSFL50 groups than in the control. 16S rRNA amplicon data revealed the dominance of Firmicutes, Proteobacteria, Bacteroidetes, Spirochaetota, and Verrucomicrobia phyla. The BSFL-replacement groups showed an increase in Proteobacteria, Bacteroidetes, and Spirochaetota compared to the control, but fewer Firmicutes. PICRUSt analysis indicated significant alterations in microbial function, particularly enhanced protein, carbohydrate, lipid, and energy metabolisms in the BSFL-fed group. Substituting 10% fishmeal with BSFL enhanced nutrient metabolism and gut microbiota in juvenile hybrid grouper. Further research is needed to explore factors affecting the efficacy of insect feed as a sustainable aquaculture diet. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje