| Abstrakt: |
Research on Actinomycetota species is becoming increasingly significant, not only for their ecological and taxonomic aspects, but primarily for their unique metabolites that hold potential for therapeutic uses. In this study, Actinomycetota bacteria strains were isolated from the sediments of two seagrass meadows, Thalassia hemprichii and Syringodium isoetifolium, as well as from plain sediment (without seagrasses) at Mjimwema, Tanzania, in the Western Indian Ocean. The isolates were identified through their morphological and biochemical characteristics, along with molecular techniques. Additionally, antimicrobial and cytotoxicity tests were also conducted. Fifteen different strains of Actinomycetota were isolated, and based on phenotypic and phylogenetic characteristics, they were grouped into seven different genera, namely: Micrococcus, Microbacterium, Ornithinimicrobium, Cellulomonas, Cellulosimicrobium, Luteimicrobium, and Dietzia. Results of 16S rRNA gene sequence analysis showed eight potential new species of Actinomycetota from these sediments. Genus Micrococcus was dominant in all samples, followed by Microbacterium. Furthermore, genera Ornithinimicrobium and Cellulosimicrobium appeared only in T. hemprichii sediment, while Luteimicrobium and Cellulomonas were observed in plain sediment only. To the best of our knowledge, genera Cellulomonas and Luteimicrobium are reported for the first time in marine sediment. The crude extract of the isolates exhibited antimicrobial effects against Candida albicans, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. The results revealed that sediments within seagrass meadows harbor unique Actinomycetota species capable of producing secondary metabolites with potential therapeutic uses. Therefore, additional research is necessary to thoroughly characterize the novel species and identify the chemical compounds responsible for the observed antimicrobial activity and bioprospecting. [ABSTRACT FROM AUTHOR] |
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