| Autor: |
OKAFOR, Michael Tochukwu, OKOLI, Uzoamaka Adaobi, NUBILA, Nkoyo Imelda, OKOYE, Kingsley Chukwuebuka, EBIDO, Chike Chukwuenyem, OKPE, Aron Chukwuemeka, CHIJIOKE, Chioli Pascal, UDEINYA, Iroka Joseph |
| Předmět: |
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| Zdroj: |
Animal Research International; Dec2024, Vol. 21 Issue 3, p5864-5870, 7p |
| Abstrakt: |
Deseeded oil palm fruit head (DOPFH) is commonly used to prepare food delicacies native to the Southeast part of Nigeria. Three fractions: DOPFH-A, B and C were derived from the filtrate of DFOP ash by fractional crystallization. DOPFH-B is an alkaline substance with a pH of 10.9. To determine the lethal dose (LD50) of DOPFH-B in mice, Lorke's method was compared with the up-and-down procedure (UDP). The tests were carried out in male and female mice which involved subcutaneous and oral administration. In Lorke's method, female and male mice were divided into three groups (n = 3) for subcutaneous and oral administration. Varying doses; 10, 100 and 1000 mg/kg were given. In the second phase, two groups (n = 3), were administered 2000 and 5000 mg/kg subcutaneously and orally. For UDP, 5000 mg/kg was administered subcutaneously (SC) to each male and female mouse in the limit test. In the main test, 7 and 12 male mice; 7 and 8 female mice were treated subcutaneously and orally with UDP doses of 550, 1750, 2000 and 5000 mg/kg. Using Lorke's method, LD50 of 3250 mg/kg was obtained for both female and male mice by both routes. UDP estimated LD50 of 2100 mg/kg for females and LD50 is greater than 2100 mg/kg for male mice administered subcutaneously. LD50 for the orally administered female and male mice was given 5000 mg/kg. UDP and Lorke's method did not highlight differential sensitivities of male and female mice to DOPFH-B. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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