| Autor: |
Rahdari, Tahereh, Ghafouri, Hossein, Ramezanpour, Sorour, Mahdavimehr, Mohsen, Asghari, Seyed Mohsen |
| Zdroj: |
Frontiers in Biomedical Technologies; 2025 Supplement, Vol. 12, p1-1, 1p |
| Abstrakt: |
Background: The application of tumor-targeted ligands is a common strategy to improve the specificity cancer detection. In this study, superparamagnetic iron oxide (Fe3O4) was prepared as a negative contrast agent in Magnetic Resonance Imaging (MRI) and loaded into a Solid Lipid Nanoparticle (SLN). Then, a tumor-targeting peptide derived from human endostatin was conjugated to the surface of SLN. The aim of this research is to synthesize and characterize a diagnostic nanoparticle to specifically target the murine 4T1 breast tumor model and enhance MRI contrast. Materials and Methods: Fe3O4 was prepared using the coprecipitation method. SLN containing Fe3O4 were synthesized using the microemulsion technique. The peptide was covalently coupled to the carboxyl groups of SLN by its N-terminal. The in vitro characteristics of the nanoparticle were evaluated by Dynamic Light Scattering (DLS), zeta potential, Vibrating Sample Magnetometer (VSM), X-ray Diffraction (XRD), Fourier-transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). The cytotoxicity of the nanoparticle was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and the tumor-targeting efficiency was measured by MRI in vivo and ex vivo histopathology. Results: The results showed that the nanoparticle was successfully prepared. The nanoparticles' mean size was 315 nm, their Polydispersity Index (PDI) was 0.3, and peptide-uncoated nanoparticle showed more negative zeta potential than peptide-coated ones. VSM confirmed the magnetic property of Fe3O4. The crystal structure of Fe3O4 and its incorporation into SLN were confirmed by the XRD patterns. FTIR spectroscopy indicated the formation of peptide-coated Fe3O4-loaded SLN. SEM confirmed that nanoparticles are spherical and uniform. The cytotoxicity assay demonstrated that it had appropriate cytotoxicity for 4T1 and MCF7 cells. The targeting efficiency was confirmed via MRI of 4T1 tumor-bearing Balb/c mice. The histopathology data confirmed that, in contrast to controls (SLN containing Fe3O4), peptide-conjugated Fe3O4-loaded SLNs specifically accumulated in tumors. Conclusion: We successfully developed peptide conjugated-magnetic SLNs that can be used for the detection of breast tumors through magnetic resonance molecular imaging. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
