| Autor: |
Akıncı, Dilara, Akkuş, Murat, Türe, Zeynep, Aydeniz Acar, Gül Ebru, Ölmez, Mehmet, Yıldırım, Esma, Yıldızhan, Eda, Akıncı, Mehmet Emin |
| Zdroj: |
Journal of Drug Delivery & Therapeutics; Dec2024, Vol. 14 Issue 12, p27-31, 5p |
| Abstrakt: |
Objectives: The main aim of this study was to investigate the effect of Ceratonia siliqua L (carob) plant added to the culture medium to reduce the negative effect on sperm parameters as a result of cryodamage occurring in sperm during human sperm cryopreservation. Materials and methods: Semen samples obtained with consent from 50 infertile men aged 25-40 years were subjected to spermiogram test. Spermatozoa were divided into normospermia and asthenospermia. Semen was washed with gradient method to separate spermatozoa according to motility and vitality with the least risk of cotamination. The groups were divided into 6 groups. normospermic group with gradient method, normozoospermic group with gradient method and freeze-thawed, normozoospermic group with gradient method and freeze-thawed and Ceratonia siliqua L., normozoospermic group with only gradient method, asthenozoospermic group with gradient method, asthenozoospermic group with gradient method and freeze-thawed, asthenozoospermic group with gradient method and freeze-thawed and added Ceratonia siliqua L. Semen samples were frozen at -196 °C with liquid nitrogen and stored in a nitrogen tank. Then the samples were thawed at 37 °C and vitality test was performed with eosin Y stain in all groups. Results: In order to investigate the effect of freeze-thawing on sperm parameters, only semen samples washed by gradient method were compared with the frozen and thawed group without the addition of Ceratonia siliqua L. Cryopreservation had a significant (p>0.05) negative effect on all sperm parameters. In the investigation of the effect of Ceratonia siliqua L. on cryodamage, sperm vitality parameter was compared in the groups in which Ceratonia siliqua L. was added to the incubation medium in vitro. Ceratonia siliqua L. concentration was adjusted as 0.01%. A positive difference was observed between the groups to which Ceratonia siliqua L. was added compared to the groups to which Ceratonia siliqua L. was not added. Conclusion: According to the results of this study, the negative effects of sperm freezing on sperm vitality are clearly demonstrated. Considering the positive differences in the groups in which Ceratonia siliqua L. was added in vitro, we think that Ceratonia siliqua L. will contribute to male infertility. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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