| Abstrakt: |
Biofilms are complex substances secreted by microorganisms, capable of adhering to various surfaces, leading to contamination of living tissues, environments, industrial settings, and medical equipment. They play a significant role in the emergence of antibiotic-resistant bacteria. This study aimed to evaluate the production of the enzymes amylase, cellulase, and xylanase, as well as the antibiofilm activity against pathogenic bacteria of Streptomyces sp. WPN31, isolated from the rhizosphere of pandanus palm (Pandanus amaryllifolius). Based on the 16S rRNA results, WPN31 exhibited the highest similarity to Streptomyces griseicoloratus (98.80%), a novel streptomycete isolated from soil in cotton fields of China. However, Streptomyces sp. WPN31 was able to produce extracellular enzymes, including amylase, cellulase, and xylanase, at both 37 °C and 40 °C. The evaluation of biofilm inhibition using WPN31 supernatant revealed its ability to prevent biofilm formation in both Staphylococcus aureus and Pseudomonas aeruginosa. Therefore, this study represents the initial report on the antibiofilm activity of Streptomyces sp. WPN31, suggesting its potential application in various industries. [ABSTRACT FROM AUTHOR] |
