Changes in the Heterogeneity of MSC Subpopulations During Replicative Senescence as Seen from Single-Cell Speed Measurements.

Autor: Lukacheva, A. V., Gorb, A. I., Musorina, A. S., Kriger, D. V., Poljanskaya, G. G., Bobkov, D. E.
Zdroj: Russian Journal of Bioorganic Chemistry; Dec2024, Vol. 50 Issue 6, p2509-2518, 10p
Abstrakt: Objective: To examine the distribution of motility metrics of human mesenchymal stem cells (MSCs) and immortalized cell line in long-term 2D culture. Methods: We used single-cell motility tracking to analyze changes in the motility metrics (average speed, distance, track length). The study compared three cell lines: two lines of MSCs that undergo replicative senescence (DF-2, MSCWJ-1), and a third line of immortalized fibroblast-like cells with an unlimited lifespan (XP12RO). Results and Discussion: The results revealed that replicative senescence in MSCs is associated with an alteration in the distribution of observed motility metrics, which is expressed as a change from a bimodal distribution in young cells to a unimodal distribution in old cells. The distribution of motility metrics in immortalized cells did not change throughout long-term cultivation. Conclusions: Subpopulation heterogeneity decreases as MSCs undergo replicative senescence. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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